Journal: PLoS ONE
Article Title: Efficacy and Mechanism of Angiotensin II Receptor Blocker Treatment in Experimental Abdominal Aortic Aneurysms
doi: 10.1371/journal.pone.0049642
Figure Lengend Snippet: After sacrifice, aortae were fixed, sectioned and underwent elastin staining and immunostaining for SMCs (SMC α-actin), macrophages (MAC2) and blood vessels (CD31). A–K: Residual medial elastin (A–E) and mural SMCs (F–K) in telmisartan (B, H), irbesartan (C, I), fluvastatin (D, J), doxycycline-treated (E, K) or control mice (A, F). L–V: macrophages (L–P) and endothelial cells (neovessels) (R–V) in telmisartan- (M, S), irbesartan- (N, T), fluvastatin- (O, U) or doxycycline- (P, V) treated mice or untreated control mice (L, R). Representative images, 4–5 mice in each group. Magnification: x200 in A–E, x100 in F–V.
Article Snippet: In brief, the PBS-rehydrated sections were incubated with a rabbit anti-mouse SMC α-actin polyclonal antibody (Laboratory Vision, Fremont, CA), a rat anti-mouse MAC2 mAb (M3/38, Cedarlane Laboratories, Burlington, Ontario, Canada), a rabbit anti-mouse CD31 polyclonal antibody (Laboratory Vision, Fremont, CA), or a species and isotype-matched negative control antibody.
Techniques: Staining, Immunostaining